*	Connecting to chain bus kernel module...
*	Connected!
*	CVM link already established.
*	Executing user-supplied startup script /home/users/charl/.chatstart.conf
*	Looking up chain://chat.zmdh63z2aoocurdid27p3ti2fddfmjxx4vk3kcni2i
*	Connected [.50932 uCC/kbps]
*	Capabilities supported: away-notify file-transfer forum-search clink-preview taxful-autonomics-hosting
*	Capabilities requested: away-notify file-transfer
*	Capabilities acknowledged: away-notify file-transfer
*	There are currently 96 user(s) talking on 14 channel(s) paying a total of 64.93105 uCC/kbps
*
*	MOTD:         ____    _    _   _ _  __   _ __ | __ )  / \  | \ | | |/ /  | '_ \|  _ \ / _ \ |  \| | ' /  | |_) | |_) / ___ \| |\  | . \  | .__/|____/_/   \_\_| \_|_|\_\  |_|               The net's largest independent plasmid repository! Genehackers welcome, Genetics Oversight Division GTFO. Rules: + No spamming + No advertising + No deanonymizing (direct surfaceweb links EVEN TO PUBMED are an instaban) + No pathogenic DNA or RNA sequences (INCLUDING unbootstrapped viral particles) + No prion coding sequences + Respect the administration ;) Your mods (gods) are:  __________________ [!!!!masterhack!!!!]  ^^^^^^^^^^^^^^^^^^       !!yui!!    !!lux_operon!!       !egene!       !retr0!        !bil! (DNAbot has also been given operator status for admin purposes) Please note that this chat is associated with the pBANK forums, and all username registration is handled through forum accounts. YOU CANT REGISTER THROUGH NICKSERV so STOP ASKING. ~MH
Nickserv	Warning: [charl] is a registered username - you are now identified as charl_2
charl_2	/msg Nickserv IDENTIFY charl *************
Nickserv	Identify success. Welcome, charl.
*	charl sets mode +i on charl
*	charl sets mode +z on charl
charl	/join #general
*	Now talking on #general
*	Channel topic: pBANK general chat, Q&A | Global rules apply | Don't ask to ask.
*	Client logging start, scrollback disabled
canislupis	lemmie check
canislupis	@bil yeah protocol says 100 millimolar
bil	What the fuck.
bil	Can you copy exactly what it says?
canislupis	yea one sec
*	rosalin has left
*	rosalin has joined
canislupis	"Prepare .1 M lithium acetate in a 25 milliliter conical and store at room temperature (20C)"
rosalin	Fucking outages.
rosalin	I swear they're engineered to hit the computer district.
bil	@lupis What does it say about the transformation mix?
canislupis	it says to use the 100 mM lithium acetate in the trafo yeah
bil	It doesn't say to use that to wash the cell pellet?
canislupis	it says to use it in the wash too
canislupis	well one of em
canislupis	first wash is acetate, seconds water
bil	This is cerevisiae you're transforming, right?
canislupis	yeast, yeah
bil	Well, I guess we've found your problem then.
bil	It doesn't have to do with the pBANK plasmid DNA you got - The LiAc is just too dilute.
bil	Try using 1M, instead of .1M LiAc in the TRAFO
*	!!!!masterhack!!!! has joined
canislupis	wont that kill the cells?
bil	It shouldn't.
bil	Give it a shot, and hit me up once you've got some results.
rosalin	And ~mysteriously~ the power ~never~ goes out for the offices across the tracks.
canislupis	ill try it and see. thanks, cya @bil
bil	Good luck lup!
*	canislupis has left
rosalin	Just for the poor sods running Internet scams on shitty library computers.
rosalin	These outages are just another arm of the trade council's crackdown on cybercrime.
rosalin	Trying to make the country more "investor friendly".
rosalin	Newsflash: The only reason you HAVE a computer industry TO invest in is because of us!
masterhack	That's what you get for living in a shit country, rose <:)
rosalin	Oh fuck off. We can't all just pump money out of the ground.
*	!!yui!! is no longer idle
yui	you pwoooomised no fights with woooosealin >//<
masterhack	Fuck, that's right. Sorry ue, I'll stop.
yui	Thank you maaaster <3333
bil	How's the circuit-bending coming along, @yui?
yui	Qwite well!
yui	I stwipped the cwoud feedback but weft network capibiwity!
yui	I thwink I'll have a working network-enabled bioweactor pwetty soon! ^u^
bil	How did you remove just the one?
yui	:?
bil	I thought it was all soldered together?
yui	Oh! I twook a software appwoach.
yui	I wemoved aaaaaawwwwwll the micwocode that talks to sigma biowogics OwO
*	yui added file "sigmaS33-300Litre-firmware-FWIXED.bin" to the download list (901.5 MB, client hosting)
DNAbot	**SCANNING NEW FILE, DO NOT DOWNLOAD UNTIL VERIFIED.**
DNAbot	**New file does not contain pathogenic DNA or RNA sequence in any known format. Proceed with caution.**
*	bil added "sigmaS33-300Litre-firmware-FWIXED.bin" to their download queue
*	rosalin added "sigmaS33-300Litre-firmware-FWIXED.bin" to their download queue
bil	Christ almighty!
*	charl added "sigmaS33-300Litre-firmware-FWIXED.bin" to their download queue
rosalin	Oh hey charl, didn"t realize you were on.
charl	Just tabbing in and out.
charl	JJ said he'd be on around now.
masterhack	You really think he's gonna show?
bil	Did you edit this manually @yui???
yui	Yes! Wots and wots of hand-tooled assembwy code! "u"
bil	Goddamn. You're pretty hardcore.
yui	Nuuuuuuuuu!!!!!!! I'm soft!!!!!  >//<
masterhack	Yep, ue's just a big marshmallow.
yui	^u^ <3
*	Guest_11468 has joined
Guest_11468	Can I asking question
Guest_11468	How do I know what enzyme to use
Guest_11468	I don't have sequencer
rosalin	@charl Do you think you could do your antibody production in yui's new bioreactor?
Guest_11468	Sorry bad english
masterhack	Enzyme? For what?
masterhack	You could be doing literally anything.
masterhack	Fucking idiot.
bil	@Guest_11468 No sequencer... Are you trying to verify a plasmid sequence?
*	!!yui!! is now idle.
Guest_11468	Got pBANK plasmid 10412 says I have to cut it with enzyme to check
Guest_11468	Yes, @bil
Guest_11468	Doing "gel electrophoresis"
charl	@rosalin Possibly.
bil	Looking at that plasmid"s thread, it seems like ClaI is a good choice.
bil	You should get three bands.
Guest_11468	thanks @bil
bil	No problem. Some advice:
bil	Get a direct CVM connection set up.
rosalin	@charl You've got to scale up production. Everyone else's suck.
bil	Don"t connect through a surfaceweb service like you are now.
bil	Hypothetically, GOD could pull their records and deanonymize you.
Guest_11468	Thanks.
Guest_11468	I not genehacker, thank you @bil for time and wise wisdom
bil	What are you using p10412 for?
*	Guest_11468 has left
masterhack	Why can't these fuckers learn English?
masterhack	It's been the language of the internet for a century now.
masterhack	Yet they still can't conjugate a verb to save their life.
rosalin	Now gather round everyone.
rosalin	Take a look at this textbook example of linguistic imperialism!
rosalin	Let me tell you all the ways in which MH"s comment stereotypes non native speakers,
*	!!lux_operon!! has joined.
rosalin	Enforces a broken post-post-colonial value system, oppresses the technically illiterate,
rosalin	And just generally perpetuates the economic-might-makes-right attitude that is currently killing the planet!
masterhack	If you want to get ahead in life, you need to know English.
masterhack	That's a fact.
masterhack	No amount of wishy-washy "won't somebody think of the idiots" bleeding heart bullshit is going to change that.
lux_operon	O god, not this shit again.
lux_operon	@yui hmu w/ firmware l8er
*	!!lux_operon!! has left.
yui	MASTER, WOSALIN
yui	pwwwwwwwweeeeese stop fighting >>>XPPP
yui	Or take it to #pawaticks
rosalin	What? What channel is that?
rosalin	Oh, "politics"
rosalin	That really gets mangled.
*	j.johnson has joined
charl	Welcome back.
rosalin	Hello again! Do you have the data?
j.johnson	Some wireman, please check my connection
j.johnson	I need to make sure GOD can't see I"m here.
masterhack	What the hell could a wireman do?
masterhack	"Yeah, go down the hallway on your left and GOD won"t see you"
masterhack	Idiot.
j.johnson	Or computerman, I don't know the slang.
bil	There isn't much we can see on our end.
bil	We could guess if you were using a surfaceweb service from your username, but that's about it.
j.johnson	Is there any way you can check if GOD can see me?
bil	I can ask you some questions about how you're connected, and guess.
j.johnson	Alright, shoot.
bil	Are you connecting on a windows machine like last time?
j.johnson	No, I bought a new laptop and installed Linux SilentRunner
masterhack	MemeOS, got it
j.johnson	Did I mess up?
bil	No, runner's fine. It"s just used by a lot of script kiddies is all.
bil	What chainchat client are you using?
j.johnson	Zchat
bil	Ok, and where are you connecting from?
j.johnson	I drove out into the slums and connected with a phone hotspot.
*	rosalin has left
*	rosalin has joined
rosalin	FUCK
j.johnson	Sorry, I should have connected at a cafe like you suggested
rosalin	No, It's just another outage.
j.johnson	Oh
bil	What matters is that you're not on GG's network.
j.johnson	Ok
bil	Alright, while I can't say for sure, you're probably safe from the eyes of GOD.
bil	Though you probably should have picked a less identifiable username.
j.johnson	Shit
bil	Don't worry about it.
j.johnson	Ok
j.johnson	I have the files
charl	Upload them.
j.johnson	Ok
j.johnson	Ok
j.johnson	Here I go
*	j.johnson added file "xxxxxx_xxx_xxxxxxxxa0xCC.zip" to the download list (475.5 GB, client hosting)
DNABot	**SCANNING NEW FILE, DO NOT DOWNLOAD UNTIL VERIFIED.**
DNABot	**Recognized archive format ZIP, extracting...***
DNABot	**WARNING, FILE "xxxxxx_xxx_xxxxxxxxa0xCC.zip" CONTAINS GG BIS FORMAT DNA AND RNA DATA**
DNABot	**Running nucleotide BLAST against known pathogens...**
DNABot	**New file does not contain pathogenic DNA or RNA sequence in any known format. Proceed with caution.**
*	charl added "xxxxxx_xxx_xxxxxxxxa0xCC.zip" to their download queue
*	masterhack added "xxxxxx_xxx_xxxxxxxxa0xCC.zip" to their download queue
masterhack	Holy shit, there it is.
*	bil added "xxxxxx_xxx_xxxxxxxxa0xCC.zip" to their download queue
*	yui added "xxxxxx_xxx_xxxxxxxxa0xCC.zip" to their download queue
masterhack	He came through.
bil	Jesus Christ.
*	rosalin added "xxxxxx_xxx_xxxxxxxxa0xCC.zip" to their download queue
yui	<:O
j.johnson	i just violated a General Genetics NDA
j.johnson	I"m an employee
j.johnson	I rent my apartment and buy my groceries from GG
j.johnson	What the hell am I going to do now?
rosalin	You"re going to keep living just as you were before.
j.johnson	But what if they find out??
rosalin	If bil says you're safe, you're safe.
rosalin	We"ll add the data to the pBANK repository, and we"ll list the source as "Anonymous"
j.johnson	Can you delete these messages?
rosalin	The chat?
j.johnson	Yes, the messages here.
rosalin	They"ll actually be autodeleted.
rosalin	This chainchat server doesn't store scrollback.
rosalin	Convienient, eh?
masterhack	Everyone's logging to disk.
j.johnson	I just couldn't take it anymore
j.johnson	Mpox is preventable
j.johnson	It's been preventable since the late 60's
j.johnson	But GG has the patents
j.johnson	They're the only ones making vaccine at scale, and they're not making enough.
rosalin	Fucking naturally.
rosalin	Just scarce enough that the price stays high without justifying the investment required to undercut them.
rosalin	No tactic is too dirty, no market manipulation too blatant for the biological-industrial complex!
j.johnson	I
j.johnson	Oh my god
j.johnson	I can't believe I just gave privileged data to fucking genehackers
rosalin	Before you uploaded that file,
rosalin	Anyone here could whip a few dozen doses of Mpox vaccine up.
rosalin	Inoculate a few people, maybe.
rosalin	Not enough to make a real difference.
rosalin	Now, we can produce vaccine at scale.
masterhack	Assuming we can get it to work, that is.
rosalin	You've really done something remarkable today, jj.
rosalin	You've saved thousands from disfigurement and death.
charl	It's no good.
yui	:?
charl	I suspected as much when you said you only had third level clearance.
charl	I'm not an expert on GG's corporate hierarchy, but that seemed too low to have access to Mpox stuff.
charl	There"s way too much higher-order RNA structure in this file.
charl	This looks like a virus.
rosalin	I mean it is a virus, essentially. Just an inactivated one.
charl	Check the sequence data.
rosalin	This doesn't look so bad.
charl	Look at the tertiary structure of the RNA.
charl	993247_a.ggrna in particular.
rosalin	What?
rosalin	Oh god fucking damnit.
bil	That's a lot of ribozymes.
j.johnson	Ribozymes?
rosalin	What the hell is this shit, Johnson??
masterhack	So all along, he was just trying to get us to kill ourselves.
j.johnson	What???
rosalin	You really had me going there, asshole.
j.johnson	I just downloaded the Mpox stuff and zipped it!!!
yui	OOOOOoooo! "gizmocat"
yui	Your password's so cuuuuute, Jeremy! \  (^ w ^ ) /
j.johnson	WHAT THE FUCK
yui	Megapik got hacked wast year, you know.
yui	You weeeeeally shouldn't weuse passwords between sites... `n`
yui	ESPECIWLLY for your GG citizenship wogin...
j.johnson	I JUST UPLOADED THEFILES I DIDNT KNOW
bil	Yui, stop.
bil	Stop whatever stunt you're pulling.
bil	I'm very impressed, but we can't jump to any conclusions.
rosalin	bil's right. If JJ were an agent of GOD, his GG account would have been better secured.
yui	<_<
masterhack	Aw. I was looking forward to seeing ue wreck someone's shit.
yui	<3
charl	Are you people seeing this virus???
charl	It's fantastic!
charl	The envelope and nucleocapsid are pretty standard shit,
charl	But the RNA is almost completely novel!
charl	It's like something that came out of a high throughput pathogenicity screen, where they just try everything.
charl	But it's human targeting, which would suggest that it's engineered.
charl	Since obviously you wouldn't evolve a virus in human subjects.
roslalin	Wouldn't put it past sigma.
charl	It would be too expensive, regardless of ethics.
j.johnson	I just saw a directory labeled "Mpox vaccine" on my branch's server, and I gave it to you, OK???
yui	Awwww, you changed your paaasword jeeeeeeewameeeeeee....
masterhack	Even if he's not a GOD agent, JJ here just tried to kill us.
charl	We have no idea if it's lethal.
rosalin	Really??
charl	Yeah! I have no idea what its effect on the human body would be!!!!
charl	It's got some motifs that look like they'd interact with liver cells, but beyond that all bets are off!
charl	Isn't it fantastic!!
rosalin	Is is possible that JJ just got his hands on something weird by mistake?
charl	No, this was clearly designed to slip by our DNAbot.
charl	The bot just checks sequences against known pathogens, so since this is completely unknown, it slipped through!
masterhack	Yes charl, we all know how DNAbot works.
masterhack	None of us have had any brain hemorrhages since you explained it last time.
rosalin	Oh fuck off. You're just sore you had to get charl to set it up.
charl	I just like to think out loud.
j.johnson	So where the hell did this virus come from??
bil	It was a setup.
bil	GG probably knew you were upset about the availability of Mpox vaccine, and so they slipped you the virus.
bil	With the goal of killing or flushing out some genehackers.
bil	Did you make any posts on social media about your concerns regarding the vaccine?
j.johnson	Oh
j.johnson	Oh fuck
j.johnson	On megapik.
j.johnson	But wait,
j.johnson	I did what they wanted.
j.johnson	So, maybe, they don't have any problems with me??
bil	They know you're willing to give privileged information to genehackers, so they'll likely fire you.
masterhack	Probably press charges too - so they can have some deniability.
bil	I'd leave the country.
j.johnson	fuck
j.johnson	FUCK
j.johnson	Why did I do this
j.johnson	I crawled my way into a tech job with a decent salary, and now it's all gone
j.johnson	My girlfriend's pregnant, all my money is in GG cash,
j.johnson	What the hell am I going to do???
bil	I have some friends who might be able to help. Let's take this to a PM.
j.johnson	I
j.johnson	Ok
*	!bil! has left.
*	j.johnson has left.
rosalin	What kind of scumbag comes up with a tactic like that?
charl	I have to synthesize it.
rosalin	The virus?
charl	In silico isn't cutting it, I need to see how this thing ticks.
masterhack	Wait, did you actually have a brain hemorrhage? I was joking, charlie!
charl	Oh, it's not that dangerous.
charl	I've got a cheap little tabletop reactor I can stick in quarantine use and burn afterwards.
yui	I twied alweady. Its a biiiiit two cooooompwicated for a widdle desktop weactor like that. O_o
masterhack	You tried????
yui	Of course! charl is wight, this viwus is suuuuuuuper cool B)
charl	I think my reactor can handle it.
charl	I've got a couple other things I can try regardless.
charl	Alright, I gotta go.